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KMID : 0387820030100020262
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Clinical Pediatric Hematology-Oncology
2003 Volume.10 No. 2 p.262 ~ p.268
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The Effect of Vasoactive Intestinal Peptide on Cord Blood CD34(+) Cells
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Yeo Mi-Kyong
Bae Hwa-Young
Hur Min-Kyu
Ra Jae-Sun
Han In-Seob
Kim Min-Keong
Kim Soon-Ki
Park Sang-Kyu
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Abstract
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Purpose: We investigated the expression of vasoactive intestinal peptide (VIP), VIP receptor 1 (VPAC1), VIP receptor 2 (VPAC2) genes in the human umbilical cord blood CD34 cells, and the ability of VIP to stimulate human primitive as well as monopotent hematopoietic progenitors.
Methods: We isolated RNA from umbilical cord blood CD34 cells, and then performed RT-PCR, and sequencing. The umbilical cord blood CD34 cells were cultured with the various concentrations of VIP for burst-forming unit of erythrocyte (BFU-E), colony-forming unit of granulocyte/monocyte (CFU-GM), colony-forming unit of graulocyte/erythrocyte/monocyte/megakaryocyte (CFU-GEMM), and colony-forming unit of megakaryocyte (CFU-Mk).
Results: The RNA coding for VPAC1 was detected in human umbilical cord blood CD34 cells. VIP significantly stimulated the growth of CFU-GEMM and CFU-Mk.
Conclusion: The present results suggest that VIP is an important neuropeptide in the early proliferation of human primitive as well as megakaryocyte progenitors.
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KEYWORD
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Vasoactive Intestinal Peptide, Vasoactive Intestinal Peptide Receptor, Umbilical cord blood, CD34(+) Cells, CFU-GEMM, CFU-Mk
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